Discrimination between sheep-associated and wildebeest-associated malignant catarrhal fever virus by means of a single-tube duplex nested PCR
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Discrimination between sheep-associated and wildebeest-associated malignant catarrhal fever virus by means of a single-tube duplex nested PCR.
A single-tube duplex nested polymerase chain reaction (sdn-PCR) was developed for the detection of and discrimination between ovine herpesvirus-2 (OvHV-2) and alcelaphine herpesvirus-1 (AIHV-1). These viruses respectively cause sheep- and wildebeest-associated malignant catarrhal fever (SA-MCF and WA-MCF). In the first step of the sdn-PCR, two primers with high annealing temperatures based on c...
متن کاملMalignant catarrhal fever in pastoral Maasai herds caused by wildebeest associated alcelaphine herpesvirus-1: An outbreak report
An outbreak of malignant catarrhal fever (MCF), a fatal viral disease in indigenous Tanzanian shorthorn zebu in Ngorongoro district of Tanzania during the period of June 2004 has been described. The disease was diagnosed by clinical, post mortem findings and the virus was identified using molecular characterization study. The history and clinical features included pyrexia, cornel opacity, nasal...
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Development of control measures for the gammaherpesviral disease of cattle known as sheep-associated malignant catarrhal fever (SA-MCF) has been hampered by a lack of accurate diagnostic tests either for the causative virus or for antibody against that virus. A recently developed competitive-inhibition enzyme-linked immunosorbent assay (CI-ELISA) for the detection of antibody to malignant catar...
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The epidemiology of wildebeest-derived malignant catarrhal fever in South Africa differs from the worldwide accepted pattern. Here the occurrence of the disease is often not related to close contact between cattle and wildebeest, and most cases are observed during late winter and spring, when wildebeest calves are 8-10 months old. This is in contrast to the situation in Kenya and Tanzania, wher...
متن کاملmalignant catarrhal fever in pastoral maasai herds caused by wildebeest associated alcelaphine herpesvirus-1: an outbreak report
an outbreak of malignant catarrhal fever (mcf), a fatal viral disease in indigenous tanzanian shorthorn zebu in ngorongoro district of tanzania during the period of june 2004 has been described. the disease was diagnosed by clinical, post mortem findings and the virus was identified using molecular characterization study. the history and clinical features included pyrexia, cornel opacity, nasal...
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ژورنال
عنوان ژورنال: Onderstepoort J Vet Res
سال: 2005
ISSN: 2219-0635,0030-2465
DOI: 10.4102/ojvr.v72i4.184